Journal: bioRxiv

Article Title: A Photonic Biosensor-Integrated Tissue Chip Platform for Real-Time Sensing of Lung Epithelial Inflammatory Markers

doi: 10.1101/2022.06.21.497028

Figure Lengend Snippet: a) Design in GDS (Graphic Design System) format used to produce photolithographic masks for the photonic chip. The overall footprint is 5.8 × 4.0 mm, with 2 banks of 7 × 2 ring resonators, each with a total diameter of 300 μm including the trench (open area in the top layer of SiO 2 allowing direct contact between the ring resonator and analyte-containing solution). One temperature control ring (under oxide) is also provided in each bank. b) A photograph of the chip after fabrication and dicing. c) Functionalization chemistry using a succinic anhydride-bearing silane. d) Photomicrograph of a ring bank, with the antibody layout superimposed. e) An image acquired by the microarrayer immediately after spotting shows reproducible spotting of antibody solutions and StabilGuard over the rings. The dark spots are ∼250 pL drops settled in the ring trenches.

Article Snippet: After silanization, antibody solutions were printed on the rings using a Scienion SX SciFLEXarrayer piezoelectric microarrayer equipped with a PDC-60 capillary nozzle.

Techniques: Control

Journal: BMC Genomics

Article Title: A highly sensitive and specific system for large-scale gene expression profiling

doi: 10.1186/1471-2164-9-9

Figure Lengend Snippet: Comparison of microarray results between duplicated samples . A . A scatter plot of the microarray intensities of a sample with 100 NCI/ADR-RES cells were plotted against those of a duplicated 100-cell sample, and B , a scatter plot in A after replacing the data of the latter 100-cell sample with data of a 10,000-cell sample from the same cell line. Microarray intensities shown in the figure are the results after baseline subtraction. Both plots are on the log scale.

Article Snippet: Slides were dried by centrifugation at 1,000 rpm for 5 min with a slide holder in a GS-6 Beckman centrifuge, and then were baked at 140°C in a vacuum oven for 4–5 hrs (Fisher Scientific Model 280A); (2) microarray preparation: each oligonucleotide probe was mixed with the Microarray Printing Solution (GenScript, Piscataway NJ) at a 1:5 ratio (v/v) to a final concentration of 50 μM in a well of a 384-well plate.

Techniques: Comparison, Microarray

Journal: BMC Genomics

Article Title: A highly sensitive and specific system for large-scale gene expression profiling

doi: 10.1186/1471-2164-9-9

Figure Lengend Snippet: Differential gene expression in the two cell lines, MCF-7 and NCI/ADR-RES . Upper , Microarray images from the two cell lines. Lower , confirmation of the gene expression profiling results by gel assay. Quantitative results are given in Table 3. M, MCF-7, and A, NCI/ADR-RES.

Article Snippet: Slides were dried by centrifugation at 1,000 rpm for 5 min with a slide holder in a GS-6 Beckman centrifuge, and then were baked at 140°C in a vacuum oven for 4–5 hrs (Fisher Scientific Model 280A); (2) microarray preparation: each oligonucleotide probe was mixed with the Microarray Printing Solution (GenScript, Piscataway NJ) at a 1:5 ratio (v/v) to a final concentration of 50 μM in a well of a 384-well plate.

Techniques: Gene Expression, Microarray

Journal: BMC Genomics

Article Title: A highly sensitive and specific system for large-scale gene expression profiling

doi: 10.1186/1471-2164-9-9

Figure Lengend Snippet: Comparison between the results from microarray and gel assay*

Article Snippet: Slides were dried by centrifugation at 1,000 rpm for 5 min with a slide holder in a GS-6 Beckman centrifuge, and then were baked at 140°C in a vacuum oven for 4–5 hrs (Fisher Scientific Model 280A); (2) microarray preparation: each oligonucleotide probe was mixed with the Microarray Printing Solution (GenScript, Piscataway NJ) at a 1:5 ratio (v/v) to a final concentration of 50 μM in a well of a 384-well plate.

Techniques: Comparison, Microarray